The core-specific lysosomal α(1–6)-mannosidase activity depends on aspartamidohydrolase activity

The core-specific lysosomal alpha(1-6)-mannosidase activity depends on aspartamidohydrolase activity.

The substrate specificity of the core-specific rat liver lysosomal alpha(1-6)-mannosidase was investigated using mannosylated oligosaccharides and glycoasparagines. Hydrolysis of Man(alpha 1-6) linkage hydrolysis was demonstrated to follow the action of endoglycosidases, namely aspartyl-N-acetyl-beta-D-glucosaminidase and endo-N-acetyl-beta-D-glucosaminidase. The results are discussed with resp...

Expression of human lysosomal alpha-mannosidase activity in transfected murine cells and human alpha-mannosidase deficient fibroblasts.

We studied the human lysosomal alpha-mannosidase (MANB) by expressing the putative cDNA in mammalian cells, using the eucaryotic expression vector pCDE. The construct pCDE-MANB and pSV2-Neo were cotransfected into human alpha-mannosidase deficient fibroblasts and into a murine cell line and selected by culture in the presence of G418. Six G418 resistant 3T3 clones had increased alpha-mannosidas...

Lysosomal alpha-mannosidase and alpha-mannosidosis.

Lysosomal alpha-mannosidase with acidic pH optimum is ubiquitous in human tissues where is expressed in two major forms, A and B that are the product of a single gene located on chromosome 19. Mutations in the gene encoding for alpha-mannosidase cause alpha- mannosidosis, an autosomal recessive disease, resulting in the accumulation of unprocessed mannose containing oligosaccharide material. Th...

Regulation of lysosomal a-mannosidase in macrophages

h 0 1 2 3 4 ATPase activity present in the outer-membrane fraction is probably due to partial disruption of the inner membrane by the digitonin extraction procedure. It is suggested that part of the apparent ADPase activity of intact mitochondria is mediated via ATPase, presumably in conjunction with adenylate kinase. In addition, the mitochondrial-outer-membrane contains a distinct ADPase not ...

Staphylococcus aureus Activity Depends on RsbU inB